Phage display

Forfatter/Opretter:

Thomas Shafee

Attribution:

Billedet er tagget "Attribution Required", men der blev ikke angivet nogen tilskrivningsoplysninger. Attributionsparameteren blev sandsynligvis udeladt ved brug af MediaWiki-skabelonen til CC-BY-licenserne. Forfattere og ophavsmænd finder et eksempel på korrekt brug af eksempel her. her.

Kredit:

Eget arbejde

Shortlink:

https://dkwiki.dk/b/2d869

kilde:

Wikimedia Commons

størrelse:

2826 x 1798 Pixel (627003 Bytes)

beskrivelse:

Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated. 5) those genes may have random mutations introduced and used to perform another round of evolution. The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders.

Licens:

CC BY 4.0

Licensbetingelser:

Creative Commons Attribution 4.0